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Platelet Antigen Typing Panel

Platelet Antigen Typing (Level 1, 2, and 3), Platelet Typing

Test Codes

EPIC: LAB6441, Beaker: XPLAG, Blood Center of Wisconsin / Versiti: 5600

Department

Send Outs

Instructions

• If a patient has received a whole blood or platelet transfusion, wait 24 hours before drawing sample.  Please indicate on the requisition when the patient received the most recent transfusion.
• Please provide patient's platelet count on requisition, if known.

Specimen Collection Criteria

Collect:

• Whole Blood: Two Lavender-top EDTA tubes.
• Fetal Specimen: 15.0 mL of amniotic fluid, 5x10⁶ amniocytes cultured fresh, or cord blood.

Physician Office/Draw Specimen Preparation

Maintain whole blood or fetal specimens at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: Whole blood or fetal specimens, at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Maintain whole blood or fetal specimens at room temperature (20-26°C or 68-78.8°F) prior to transport.

Transport: Whole blood or fetal specimens, at room temperature (20-26°C or 68-78.8°F).

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 96 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Blood Center of Wisconsin / Versiti, Milwaukee, WI.

Performed

Performed 3 times a week.
Results are available in 7 days.

Reference Range

By report.

Test Methodology

Polymerase Chain Reaction (PCR).

Interpretation

Platelet Antigen Typing (Level 1, 2, and 3).

• Level 1 must be done before Level 2 or 3 can be ordered.
• Level 1 includes: Pl^A1/A2, Br^a/b, Bak^a antigens.
• Level 2 includes: Bak^b, Ko^a/b, Pen^a/b antigens.
• Level 3 includes: Ca^a/b antigens.

Clinical Utility

If this assay is ordered alone the clinical significance is up to the doctor. This assay is usually ordered as a panel with other tests to rule out post-transfusion purpura or neonatal alloimmune thrombocytopenia.

CPT Codes

81105, 81106, 81107, 81108, 81109, 81110, 81111, 81112.

Contacts

Last Updated

11/3/2023