Laboratory Lab results are too important to go anywhere else.

Neutrophil Antibody Screen

Granylocyte Antibody Screen

Test Codes

Blood Center of Wisconsin: BCW5102. Order as a Miscellaneous Send Out.

Department

Send Outs

Specimen Collection Criteria

Collect: Two plain Red-top tubes.

• Do not use Serum Separator Tubes.
• Send specimens for processing immediately after collection.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 5.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 5.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL) 

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Blood Center of Wisconsin, Milwaukee, WI.

Performed

Once per week.
Results available in 7-10 days.

Reference Range

Negative.

Test Methodology

Flow Cytometry.

Interpretation

By report.

Clinical Utility

This assay is used primarily to determine causes of low neutrophil counts called neutropenia. Neutropenia can be seen in newborns when the mother makes antibodies against the baby's neutrophils. This condition is called alloimmune neonatal neutropenia. Young children (6 months to 5 years) may develop an autoantibody against their own neutrophils putting them at risk for infection. This condition is called autoimmune neutropenia. Adults may also develop autoimmune neutropenia but this condition is not very common. Neutrophil antibodies can cause a severe life-threatening reaction following blood transfusion called transfusion related acute lung injury. The antibodies are found in the donor's blood. This test cannot be used to screen donors for neutrophil antibodies prior to transfusion.

CPT Codes

86021

Contacts

Last Updated

9/21/2023