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Estradiol (E2)

E₂, 17 ß-Estradiol, Estradiol Level, Estradiol (E₂)

Test Codes

Antrim #17015, EPIC: LAB5044

Department

Chemistry

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)

Contact the Laboratory for acceptability of other tube types.

Physician Office/Draw Specimen Preparation

Let SST specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection.

Preparation for Courier Transport

Transport: Centrifuged SST tube, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Rejection Criteria

Red-top tubes with serum not separated from cells within two hours of collection. 

In-Lab Processing

Let SST specimens clot 30-60 minutes. Centrifuge SST tubes or Microtainers^® to separate serum from cells. Deliver immediately to the appropriate testing station.

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gel
Room Temperature (20-26°C or 68-78.8°F): 2-4 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gel
Room Temperature (20-25°C or 68-77°F): 2-4 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2-4 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 7 days

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Deaborn Chemistry Laboratory

Royal Oak Automated Chemistry Laboratory

Performed

Sunday – Saturday, 24 hours a day.
STAT results available within 1 hour of receipt to the Royal Oak Laboratory. (Outreach Only)
Results available within 24 hours of receipt in the Laboratory.

Reference Range

Males: 11-44 pg/mL.

Females:
Follicular: 21-251 pg/mL.
Mid cycle: 38-649 pg/mL.
Luteal: 21-312 pg/mL.
Post-menopausal: ≤28 pg/mL.

Test Methodology

Chemiluminescence Immunoassay.

Interpretation

• High Estradiol serum values are seen with: Ovarian tumors, adrenal feminizing tumors, precocious puberty (female), liver disease, and male gynecomastia.
• Low Estradiol serum values are seen with: Ovarian failure and oral contraceptives.
• CAUTION: Interference by Fulvestrant

• NOTE: This assay should NOT be used to assess estradiol levels for patients undergoing Fulvestrant treatment.  An alternate method, such as chromatography, should be used.  All analogues of the estradiol molecule have the potential to cause interference/cross reactivity with the Abbott ARCHITECT Estradiol assay. If estradiol testing is required on such a patient, please order Estrogens, Serum Fractionated. The sample will then be sent to Mayo Medical Labs for testing by LC-MS.  

Clinical Utility

Measurement of estradiol is used clinically in the investigation and management of fertility disorders, gynecomastia, estrogen-producing ovarian and testicular tumors and in hyperplasia of the adrenal cortex. Serum E₂ is measured to determine the estrogen status of women, such as in some cases of amenorrhea, and as a guide to monitoring follicular development during ovulation induction and to avoid hyper-stimulation.

CPT Codes

82670
LOINC: 2243-4, 35384-7

Contacts

Last Updated

11/4/2022