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Cutaneous Immunofluorescence Antibodies (IgG), Serum

Human Split Skin, Monkey Esophagus, Pemphigoid, Pemphigus, Split-Skin Assay

Test Codes

EPIC: LAB6607, Beaker: XCUTI, MAYO: CIFS

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One plain Red-top tube.
Also acceptable: One Gold-top SST tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 2.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL)

Rejection Criteria

Lipemic specimens.

Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 2.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 14 days
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Medical Laboratories, Rochester, MN.

Performed

Monday – Friday.
Results available in 3-8 days.

Reference Range

By report.

Test Methodology

Detection of IgG Anit-Intercellular Substance (ICS) and Anti-Basement Membrane Zone (BMZ) Antibodies by Indirect Immunofluorescence Technique Using Rhesus Monkey Esophagus Substrate and Human NaCl Split-Skin Substrate.

CPT Codes

88346, 88350.
LOINC:  45178-1

Contacts

Last Updated

9/12/2023